Fig. 1

Platelet function results in patients with hereditary haemorrhagic telangiectasia (n = 22) and healthy controls (n = 20). A–C illustrates the gating strategy applied for CD62P expression. A The dot plot demonstrates the discrimination of platelets from red blood cells (RBCs) based on size and content using forward scatter and side scatter characteristics, followed by the ubiquitously expressed platelet membrane marker (CD42b) (B). The baseline expression of platelet activation markers was defined using a non-stimulated EDTA-sample (dark grey population) (C). Platelet function results are reported as dots, and the horizontal line is median for the group. D, E and F display the platelet activation in terms of the percentage of platelets positive for activated GPIIb/IIIa, CD62P, CD63, and after stimulation with agonist in comparison to the negative control sample. Results are analysed by flow cytometry after gating on platelets [14]. ADP adenosine diphosphate, TRAP thrombin receptor-activator peptide, CRP collagen related peptide