Fig. 1

Histological findings of liver biopsies in patients underwent twice liver punctures. A Patient 3: (i) first hepatic histopathology: G1–2S1–2, no significant abnormalities in the small bile duct; (ii) second hepatic histopathology: G2S4, interlobular bile duct stenosis, small bile duct hyperplasia. B Patient 5: (i-ii) first hepatic histopathology: G1–2S4, pseudolobular structure formation, interlobular bile duct epithelial edema, vacuolation, and small bile duct hyperplasia; (iii) second hepatic histopathology: G2S2, interlobular bile duct stenosis, and small bile duct hyperplasia. C Patient 19: (i-iv) first hepatic histopathology: (i) lobular structure disorder (rete); (ii) bile duct absence, CK7+, chronic bile salt stasis; (iii) mild interstitial fibrosis in the junction area; (iv) spot-like necrosis, decreased CD10 expression in some areas (vanishing cholangiography syndrome, biliary liver fibrosis, G2S1); (v) second hepatic histopathology: atrophy and loss of interlobular bile duct epithelium, typical interlobular bile duct is rare in some convergence areas, small bile duct hyperplasia is obvious, mild interlobular inflammation (G2S2)